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dimethylallyl diphosphate dmapp substrates  (Echelon Biosciences)
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Echelon Biosciences dimethylallyl diphosphate dmapp substrates
Dimethylallyl Diphosphate Dmapp Substrates, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dimethylallyl diphosphate dmapp substrates page 4 25  (Echelon Biosciences)
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Echelon Biosciences dimethylallyl diphosphate dmapp substrates page 4 25
Dimethylallyl Diphosphate Dmapp Substrates Page 4 25, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmapp substrates  (Echelon Biosciences)
93
Echelon Biosciences dmapp substrates
(A) Representative confocal microscopy images of N. benthamiana leaf cells transiently expressing the indicated GFP fusion proteins. For each construct, GFP fluorescence (green, first column), chlorophyll autofluorescence (red, second column) and merged images of the same field (green and red, third column) are shown. White arrowheads mark stromules. Bars, 10 μm. (B) LC-MS chromatograms of reaction products. Extracts of E. coli cells overproducing the indicated recombinant proteins (with an N-terminal 6x-His tag instead of their predicted plastid-targeting peptide) were incubated <t>with</t> <t>IPP</t> and <t>DMAPP</t> and the products were analyzed by LC-MS. Prenyl diphosphates were detected using mass-to-charge (m/z) ratios: 244.998 (IPP), 313.061 (GPP), 381.123 (FPP), 449.186 (GGPP) and 518.254 (GFPP). Retention times of available standards is also shown.
Dmapp Substrates, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmapp substrates/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
dmapp substrates - by Bioz Stars, 2026-02
93/100 stars
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substrates dmapp  (Echelon Biosciences)
93
Echelon Biosciences substrates dmapp
(A) Representative confocal microscopy images of N. benthamiana leaf cells transiently expressing the indicated GFP fusion proteins. For each construct, GFP fluorescence (green, first column), chlorophyll autofluorescence (red, second column) and merged images of the same field (green and red, third column) are shown. White arrowheads mark stromules. Bars, 10 μm. (B) LC-MS chromatograms of reaction products. Extracts of E. coli cells overproducing the indicated recombinant proteins (with an N-terminal 6x-His tag instead of their predicted plastid-targeting peptide) were incubated <t>with</t> <t>IPP</t> and <t>DMAPP</t> and the products were analyzed by LC-MS. Prenyl diphosphates were detected using mass-to-charge (m/z) ratios: 244.998 (IPP), 313.061 (GPP), 381.123 (FPP), 449.186 (GGPP) and 518.254 (GFPP). Retention times of available standards is also shown.
Substrates Dmapp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/substrates dmapp/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
substrates dmapp - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

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(A) Representative confocal microscopy images of N. benthamiana leaf cells transiently expressing the indicated GFP fusion proteins. For each construct, GFP fluorescence (green, first column), chlorophyll autofluorescence (red, second column) and merged images of the same field (green and red, third column) are shown. White arrowheads mark stromules. Bars, 10 μm. (B) LC-MS chromatograms of reaction products. Extracts of E. coli cells overproducing the indicated recombinant proteins (with an N-terminal 6x-His tag instead of their predicted plastid-targeting peptide) were incubated with IPP and DMAPP and the products were analyzed by LC-MS. Prenyl diphosphates were detected using mass-to-charge (m/z) ratios: 244.998 (IPP), 313.061 (GPP), 381.123 (FPP), 449.186 (GGPP) and 518.254 (GFPP). Retention times of available standards is also shown.

Journal: bioRxiv

Article Title: Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato

doi: 10.1101/2020.07.08.194076

Figure Lengend Snippet: (A) Representative confocal microscopy images of N. benthamiana leaf cells transiently expressing the indicated GFP fusion proteins. For each construct, GFP fluorescence (green, first column), chlorophyll autofluorescence (red, second column) and merged images of the same field (green and red, third column) are shown. White arrowheads mark stromules. Bars, 10 μm. (B) LC-MS chromatograms of reaction products. Extracts of E. coli cells overproducing the indicated recombinant proteins (with an N-terminal 6x-His tag instead of their predicted plastid-targeting peptide) were incubated with IPP and DMAPP and the products were analyzed by LC-MS. Prenyl diphosphates were detected using mass-to-charge (m/z) ratios: 244.998 (IPP), 313.061 (GPP), 381.123 (FPP), 449.186 (GGPP) and 518.254 (GFPP). Retention times of available standards is also shown.

Article Snippet: IPP and DMAPP substrates and FPP and GGPP standards were obtained from Echelon Biosciences.

Techniques: Confocal Microscopy, Expressing, Construct, Fluorescence, Liquid Chromatography with Mass Spectroscopy, Recombinant, Incubation